Owner - Paul Turchirollo - Pittsburghcobras@hotmail.com
| PLAYER | POS | TEAM | LEVEL | HOW ACQUIRED | |
| 1 | Adam Frazier | 2B | PIT | MLB | Round 1, 2020 free agent draft |
| 2 | Adam Kolarek | RP | LAD | MLB | Round 4, 2020 player draft |
| 3 | Alex Reyes | SP | STL | AAA | Round 3, 2014 player draft |
| 4 | Anthony Rendon | 3B | TEX | MLB | Round 2, 2010 player draft |
| 5 | Ariel Jurado | SP | TEX | MLB | Round 8, 2016 player draft |
| 6 | Bobby Bradley | 1B | CLE | AAA | Round 5, 2015 player draft |
| 7 | Brailyn Marquez | SP | CHC | AAA | Round 4, 2019 player draft |
| 8 | Bryan Mata | SP | BOS | A | Round 8, 2020 player draft |
| 9 | Bryce Ball | 1B | ATL | R | Round 9, 2020 player draft |
| 10 | Casey Sadler | RP | LAD | MLB | Round 8, 2020 player draft |
| 11 | Chris Bassitt | SP | OAK | MLB | Round 3, 2020 player draft |
| 12 | Cole Hamels | SP | ATL | MLB | Vancouver trades Cole Hamels and VAN 2-21 to Pittsburgh for Junior Guerra. |
| 13 | Danny Santana | OF | TEX | MLB | Round 2, 2020 player draft |
| 14 | Dustin May | SP | LAD | MLB | Round 3, 2019 player draft |
| 15 | Freddie Freeman | 1B | ATL | MLB | Round 1, 2009 player draft |
| 16 | Hyun-il Choi | SP | LAD | R | Round 10, 2020 player draft |
| 17 | Jake Junis | SP | KC | MLB | Round 5, 2018 player draft |
| 18 | Jordan Lyles | SP | TEX | MLB | Pittsburgh claims Jordan Lyles and releases Luken Baker. |
| 19 | Jordon Adell | OF | LAA | AAA | Round 4, 2017 player draft |
| 20 | Josh Naylor | 1B | SD | MLB | Round 3, 2016 player draft |
| 21 | Justin Upton | OF | DET | MLB | Round 1, 2004 expansion draft |
| 22 | Ke'Bryan Hayes | 3B | PIT | AAA | Round 1, 2016 player draft |
| 23 | Kyle Funkhouser | SP | DET | AAA | Pittsburgh trades Ben Revere and Pittsburgh #7 (2017) to Ottawa for Brooklyn #1 (2017), Ottawa #3 (2019), Ryan McMahon (PTBNL) and Kyle Funkhouser (PTBNL) |
| 24 | Liam Hendriks | RP | OAK | MLB | Round 1, 2020 player draft |
| 25 | Luke Voit | 1B | NYY | MLB | Round 1, 2019 player draft |
| 26 | Malcolm Nunez | 3B | STL | A | Pittsburgh trades Miguel Rojas and PIT 9 (2023) to Flagstaff for Malcom Nunez. |
| 27 | Manny Machado | SS | SD | MLB | Round 1, 2011 Player draft |
| 28 | Marco Luciano | SS | SF | A | Round 4, 2019 player draft |
| 29 | Matt Manning | SP | DET | AAA | Louisville trades Riley Pint, Matt Manning, LOU 1 (2018), LOU 2 (2018) and LON 1 (2019) to Pittsburgh for Corey Kluber. |
| 30 | Michael Chavis | 3B | BOS | MLB | Chicago trades CHI 1 (2019), CHI 5 (2018) and Michael Chavis to Pittsburgh for Craig Kimbrel |
| 31 | Mike Minor | RP | TEX | MLB | Round 3, 2018 player draft |
| 32 | Mike Soroka | SP | ATL | MLB | Round 4, 2016 player draft |
| 33 | Monte Harrison | 1B | MIL | AAA | Round 7, 2015 player draft |
| 34 | Nick Markakis | OF | ATL | MLB | Vancouver trades Nick Markakis and DUB 4 (2019) to Pittsburgh for Austin Jackson |
| 35 | Pete Alonso | 1B | NYM | MLB | Round 1, 2019 player draft |
| 36 | Peter Lambert | SP | COL | MLB | Round 10, 2016 player draft |
| 37 | Riley Pint | SP | COL | A | Louisville trades Riley Pint, Matt Manning, LOU 1 (2018), LOU 2 (2018) and LON 1 (2019) to Pittsburgh for Corey Kluber. |
| 38 | Ronald Acuna | OF | ATL | MLB | Round 7, 2016 player draft |
| 39 | Ryan Braun | OF | MIL | MLB | FA pickup 2/22/05, dropping Jose Acevedo |
| 40 | Ryan McMahon | 2B | COL | MLB | Dublin trades Ryan McMahon to Pittsburgh for Salvador Perez and PIT 3 (2020). |
| 41 | Stephen Vogt | C | ARZ | MLB | Round 5, 2020 player draft |
| 42 | Wilderd Patino | OF | ARZ | A | Round 8, 2020 player draft |
| 43 | Yadier Molina | C | STL | MLB | Dublin trades Yadier Molina to Pittsburgh for PIT 5 (2020), PIT 7 (2020) and PIT 7 (2022). Pittsburgh cuts Drew Steckenrider. |
| 44 | Zach Davies | SP | SD | MLB | Round 1, 2017 player draft |
| 45 | Zach Eflin | SP | PHI | MLB | Round 2, 2019 player draft |
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